主  题：Engineering red fluorescent proteins with improved properties for live

       cell and deep-tissue imaging

报告人：储军 教授

时  间：2015年12月07日（周一）下午14:00

地  点：医学楼402栋一楼会议室

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               欧洲杯官网

                 2015年12月1日

报告摘要：Fluorescent proteins (FPs) have been widely used as non-invasive markers or indicators in living organisms. Red fluorescent proteins (RFPs) are of particular interest because they extend the color palette for multi-channel imaging, and are ideal for deep-tissue imaging owing to low absorption by hemoglobin. However, existing RFPs suffer from relatively low sensitivity, limiting their use in detection of biochemical processes with subtle change in cells or monitoring biological events in small animals. Here we report three new RFPs obtained by structure-guided mutagenesis of far-red FP mNeptune. First, a bright red FP, mRuby3, showed the highest fluorescence intensity in cells and greatest FRET dynamic range among all RFPs when paired with green fluorescent protein. Second, a bright far-red FP, mCardinal, has a red-shifted excitation spectrum without reduction in brightness. With mCardinal, the differentiation of myoblasts and stem cells into myocytes in living mice with high anatomical detail were non-invasively visualized. Last, a bright cyan-excitable orange-red FP, CyOFP, enabled dual-color imaging and highly sensitive bioluminescence imaging in vivo.

报告人简介：

Jun Chu, Associate Professor, Shenzhen Institutes of Science and Technology. He received his Ph.D. degree in Biomedical Engineering from Wuhan National Laboratory for Optoelectronics at Huazhong University of Science and Technology in 2009, did his postdoctoral research in Jeanny Hardy lab at University of Massachusetts, Amherst from 2009 to 2010 and in Michael Lin lab at Stanford University from 2010 to 2015. Dr. Chu joined Shenzhen Institutes of Science and Technology as an associate professor and was selected “Hundred Talents Program” of the Chinese Academy of Sciences in 2015.

Dr. Chu has developed several novel fluorescent proteins (FPs) and FP-based biosensors. 1) A far-red bimolecular fluorescence complementation system (BiFC) that works very well under cell physiological conditions, which could be used for monitoring protein-protein interaction in vivo; 2) A GFP-based caspase-3 indicator (CA-GFP) with large dynamic range, which potentially can be used for visualization of cell’s spontaneous apoptosis during embryo and neuron development; 3) A brightest far-red FP, mCardinal, providing a useful tool for optical imaging in vivo; 4) A large Stokes shift orange FP (CyOFP), which can be excited together with GFP using single wavelength, allowing simultaneous visualization of two molecular events under two photon excitation in vivo; and 5) A green FP-red FP based FRET (fluorescence resonance energy transfer) pair (mClover3-mRuby3) with large dynamic range, which is useful for detecting weak or transient biochemical responses in living cells. He has first-authored and co-authored more than 15 journal and conference articles including one Nature Methods, 2 U.S. patents and one invited book chapter.