Leshuai Zhang, Ph.D.

School of Radiation Medicine and Protection (SRMP) and School of Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Suzhou, Jiangsu 215123, China

Cell: +86 13913193240    E-mail: zhangls@suda.edu.cn

Education                              

Ph. D., Comparative Biomedical Sciences                 Aug 2005 – May 2010

Center for Chemical Toxicology Research and Pharmacokinetics, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA  

Thesis: Toxicity of Nanomaterials and Mechanisms of Endocytic Pathways    

M. S., Molecular Biology                                               Sept 2002 – June 2005

Department of applied Biology, East China University of Science and Technology &

Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai, China

Thesis: Yeast Two Hybrid of MST1 and Membrane Translocation Signaling of Dshevelled in Wnt Signaling

B. S., Biochemistry                                                       Sept 1998 – June 2002

Department of applied Biology, East China University of Science and Technology

Thesis: Eps15 Gene Cloning, Protein Purification and Associated Function

Research Experience                            

February 2014 – Present, Professor in School of Radiation Medicine and Protection (SRMP), Soochow University, Suzhou, Jiangsu Province, China

Research Interests:

· Influence of polysaccharides from traditional medical herbs on the function of dendritic cells

· Enhanced drug side effects when using nanoparticle for drug delivery

December 2012 – January 2014, PostDoc (from December 2012) and Research Assistant Professor (from October 2013) in the Nanotechnology Innovation Center of Kansas State University.

Research Interests:

· Food safety (toxicity) on primary hepatocytes

· Nanocorona and Nanotoxicology studies

July 2012 – July 2013, Consultant, Shanghai Biowing Applied Biotechnology Co., LTD

Research Interests:

· Biomarker selection for side effect detection of chemotherapy for tumors

June 2010 – June 2012, Research Fellow in the Division for Drug Safety Research, Center for Drug Evaluation and Research, Food and Drug Administration, supported by the Oak Ridge Institute of Science and Education Fellowship Program. Under the supervision of Dr. Rodney Rouse and Dr.Thomas Colatsky.

Research Description:

· Drug induced pancreatitis in vivo, biomarker evaluation and toxicity mechanisms

· Nanoparticle toxicity prediction in vitro

· Calcium signaling in drug induced cardiovascular injury

Aug 2005 – June 2010, Graduate Research Assistant, Center for Chemical Toxicology Research and Pharmacokinetics, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina. Under the supervision of Nancy A. Monteiro-Riviere.

Research Description:

· Quantum dot nanoparticle penetration and absorption in skin

· Cytotoxicity of nanoparticles via MTT/Cell Titer Blue/Cell Titer 96AQ/LDH assays, live/dead fluorescence markers and apoptosis/necrosis markers, inflammatory factors release and reactive oxygen species (ROS).

· Nanoparticle cellular uptake and mechanisms by human epidermal keratinocytes, dendritic cells and mesenchymal stem cell derived adipose cells

2003 – 2005, Graduate Research Assistant, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai, China. Under the supervision of  Lin Li.

Research Description:

· Dishevelled as a membrane protein plays an important role in Wnt signal pathway

· Salvador interaction with MST1 and regulates its phosphorylation

· Human kinase siRNA plasmid construction

· Protein crystallization of G protein-coupled receptor kinases  

2001 – 2002, Undergraduate intern, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai, China. Under the supervision of Lin Li.

Research description:  

· Gene cloning and protein purification of G protein coupled receptor adaptor eps15/eps15R

Publications  

Zhang LW, Koci J, Brett J, Riviere JE, Monteiro-Riviere NA. 2015. Safety Assessment of Potential Food Ingredients in Canine Hepatocytes. Food and Chemical Toxicology. doi: 10.1016/j.fct.2015.02.003

Rouse RL, Zhang L, Shea K, Zhou H, Xu L, Sharron S, Rosenzweig B, Zhang J. 2014. Extended Exenatide Administration Enhances Lipid Metabolism and Exacerbates Pancreatic Injury in Mice on a High Fat, High Carbohydrate Diet. PLOS ONE. DOI: 10.1371/journal.pone.0109477

Tobin GA, Goodwin D, Zhang J, Stewart S, Knapton A, Bancos S, González C, Zhang LW,  Lawton MP, Enerson BE, Weaver JL. 2014. Phosphorylation of eNOS by the phosphodiesterase-4 inhibitor CI-1044 and its role in vascular injury. Toxicologic Pathology 42, 709-724

Zhang LW, Shea KI, Xu L, Stewart S, Zhang J, Rouse RL. 2014. Autophagy in pancreatic acinar cells in caerulein treated mice: Immunolocalization of related proteins and their potential as markers of pancreatitis. Toxicologic Pathology 42, 435–457.

Zhang LW, Monteiro-Riviere NA. 2013. Use of confocal microscopy for nanoparticle drug delivery through skin. Journal of Biomedical Optics. 18(6):061214

Zhang LW, McMahon Tobin GA, Rouse RL. 2012. Oleic acid and glucose regulate glucagon-like peptide 1 receptor expression in a rat pancreatic ductal cell line. Toxicology and Applied Pharmacology  264: 274–283.

Zhang LW, B?umer W, Monteiro-Riviere NA. 2011. Cellular Uptake Mechanisms and Toxicity of Quantum Dots in Dendritic Cells. Nanomedicine. 6: 777–791.

Zhang LW, Monteiro-Riviere NA. 2010. Lectins Modulate Multi-Walled Carbon Nanotubes Cellular Uptake in Human Epidermal Keratinocytes. Toxicology In Vitro 24: 546–555.

Zhang LW, Yang J, Barron AR, Monteiro-Riviere NA. 2009. Endocytic mechanisms and toxicity of a functionalized fullerene in human cells. Toxicology Letters 191: 149–157.

Zhang LW, Monteiro-Riviere NA. 2009. Mechanisms of quantum dot nanoparticle cellular uptake. Toxicological Sciences. 110: 138–155.

Chen T, Li M, Ding Y, Zhang LW, Xi Y, Pan WJ, Tao DL, Wang JY, Li L. 2009. Identification of zinc-finger bed domain containing 3 (ZBED3) as a novel axin-interacting protein that activates WNT/beta –catenin signalling. The Journal of Biological Chemistry 284: 6683–6689.

Monteiro-Riviere NA, Inman Al, Zhang LW. 2009. Limitations and relative utility of screening assays to assess nanoparticle toxicity in a human cell line. Toxicology and Applied Pharmacology 234: 222–235.

Zhang LW, Monteiro-Riviere NA. 2008. Assessment of quantum dot penetration into intact, tape-stripped, abraded and flexed rat skin. Skin Pharmacology and Physiology 21:166 –180.

Zhang LW, Yu WW, Colvin VL, Monteiro-Riviere NA. 2008. Biological interactions of quantum dot nanoparticles in skin and in human epidermal keratinocytes. Toxicology and Applied Pharmacology 228:200 – 211.

Zhang LW, Zeng L, Barron AR, Monteiro-Riviere NA. 2007. Biological interactions of functionalized single-wall carbon nanotubes in human epidermal keratinocytes. International Journal of Toxicology 26:103–113.

Zhang LW and Zhao J. 2006. Yeast Two Hybrid of MST1 and Its Association with Salvador. Journal of East China University of Science and Technology 32, 407– 410.

Published Abstracts

Zhang LW, Monteiro-Riviere NA, Li J, Yang L. Nanoparticles exacerbate drug induced phospholipidosis. Toxicologist CD-An Official Journal of the Society of Toxicology 144, p. 416, #1942, 2015.

Monteiro-Riviere NA, Zhang LW, Veronesi B. Toxicity assessment of six titanium dioxide nanoparticles in human epidermal keratinocytes. Toxicologist CD-An Official Journal of the Society of Toxicology 138, p.212, p.820, 2014

Zhang LW, Jeffery B, Riviere JE, Monteiro-Riviere NA. Safety assessment of potential food ingredients on canine primary hepatocytes. Toxicologist CD-An Official Journal of the Society of Toxicology 138, p.420, p.1616, 2014.

Zhang LW, Zhang J, Shea KI, Tobin GA, Knapton A, Rouse RL. Acinar Cell Autophagy in Experimental Pancreatic Injury: Immunolocalization and Response of Relevant Proteins. Toxicologist CD-An Official Journal of the Society of Toxicology, 1711, p.363, 2013.

Zhang LW, Tobin GA, Rouse RL. Regulation of Glucagon-like Peptide 1 Receptor Expression in Fatty Acid Treated Pancreatic Ductal Cells. Toxicologist CD-An Official Journal of the Society of Toxicology, 2186, p.471, 2012.

Tobin GA, Stewart S, Knapton K, Zhang J, Bancos S, Zhang LW, Enerson BE, Lawton MP, Weaver JL. Regulation of endothelial cell-specific nitric oxide synthase activity by the phosphodiesterase-4 inhibitor, CI-1044. Toxicologist CD-An Official Journal of the Society of Toxicology, 1517, p.327, 2012.

Zhang LW, Monteiro-Riviere NA. Cellular uptake mechanisms and cytotoxicity of quantum dot nanoparticles in porcine dendritic cells. Toxicologist CD-An Official Journal of the Society of Toxicology, vol. 120 (S2), p.251, 1175, 2011.

Keene AM, Knapton A, Hartman NR, Peters D, Tobin G, Zhang, L, Stewart S, Xu L, Rouse R and Tyner KM. Gold nanoparticles increase the liver toxicity of acetaminophen. The Toxicologist-Annual Meeting in Society of Toxicology, #2074, p.444, 2011

Zhang L, Rouse R, Volpe DA. Exenatide and sitagliptin: Their role in autophagosome regulated toxicity in pancreatitis. The Toxicologist, #2347, p.504, 2011.

Monteiro-Riviere NA, Zhang LW, Inman AO, Samberg ME, Xia XR, Riviere JE: Safety implications  of the skin permeation of nanoparticles. The 12th International Perspectives in Percutaneous Penetration Conference, p.7, 2010.

Zhang LW, Monteiro-Riviere NA. 2010. Lectins modulate multi-walled carbon nanotubes cellular uptake in human epidermal keratinocytes. Toxicologist CD-An Official Journal of the Society of Toxicology, p.56, 259, 2010.

Monteiro-Riviere NA, Zhang LW. Mechanisms of quantum dot nanoparticle cellular uptake. 6th Key Symposium on Nanomedicine, Sweden, #38, p.74, 2009

Zhang LW, Monteiro-Riviere NA. Mechanisms of quantum dot nanoparticle uptake in a human cell line. The Toxicologist CD-An Official Journal of the Society of Toxicology,108, S-1, 877, p.181, 2009.

Inman AO, Monteiro-Riviere NA, Zhang LW. Limitations and relative utility of screening assays to assess engineered nanoparticle toxicity in a human cell line. The Toxicologist CD-An Official Journal of the Society of Toxicology,108, S-1, 875, p.181, 2009.

Monteiro-Riviere NA, Zhang LW. Evaluation of quantum dot penetration to intact, tape stripped, abraded and flexed rat skin.  NATO Advanced Research Workshop on Nanomaterials: Environmental Risks and Benefits and Emerging Consumer Products, p.15, 2008.

Zhang LW, Yu WW, Colvin VL, Monteiro-Riviere NA. Biological interactions of quantum dot nanoparticles in skin and inhuman epidermal keratinocytes. The Toxicologist CD-An official Journal of the Society of Toxicology, 102, S-1, 1027, p.211, 2008.

Monteiro-Riviere NA, Lee HA, Zhang LW, Imran M, Colvin VL, Yu W, Riviere JE. Pharmacokinetics and biodistribution of quantum dot nanoparticles in isolated perfused skin. US EPA Interagency Workshop on the Environmental Implications of Nanotechnology. p.3, 2007.

Zhang LW, Zeng L, Yang J, Barron AR, Monteiro-Riviere NA. Biological interaction of functionalized single-wall carbon nanotubes in human keratinocytes. The Toxicologist CD-An official Journal of the Society of Toxicology, 96, S-1,1403, p.290, 2007.

Monteiro-Riviere NA, Inman AO, Zhang LW, Yu W, Colvin VL, Riviere JE. Biodistribution of quantum dot nanoparticles in isolated perfused skin and in flow-through diffusion cells. The 2nd  International Nanotoxicology Conference, San Servolo Servizi, Venice, Italy, p.17, 2007.

Zhang LW, Monteiro-Riviere N. Mechanisms of Quantum Dot Nanoparticle Cellular Uptake in Dendritic Cells. The 4th Annual Nanofrontiers Symposium. Poster 98. 2013.

Non-Published Abstracts

1. Zhang LW, Monteiro-Riviere NA. Lectins modulate multi-walled carbon nanotubes cellular uptake in human epidermal keratinocytes. North Carolina State University, College of Veterinary Medicine, Annual Research Forum, Raleigh, NC, March 12, 2010.

2. Zhang LW, Monteiro-Riviere NA. Mechanisms of quantum dot nanoparticle uptake in a human cell line. North Carolina State University, College of Veterinary Medicine, Annual Research Forum, Raleigh, NC, March 6, 2009.

3. Zhang LW, Yu WW, Colvin VL, Monteiro-Riviere NA: Biological Interactions of quantum dot nanoparticles in skin and in human epidermal keratinocytes. North Carolina State University, College of Veterinary Medicine, Annual Research Forum, Raleigh, NC, March 7, 2008.

Zhang LW, Zeng L, Yang J, Barron AR, Monteiro-Riviere NA: Biological interaction of functionalized single-wall carbon nanotubes in human keratinocytes. North Carolina State University, College of Veterinary Medicine, Annual Research Forum, Raleigh, NC, March 9, 2007.

Book Chapter

Monteiro-Riviere NA, Zhang LW. 2008. Assessment of quantum dot penetration into skin in different species under different mechanical actions. In Linkov I, Steevens J (eds.): Nanomaterials: Risks and Benefits. Springer, Dordrecht, Netherlands, pp. 41-52.

FDA Internal Report

Tyner K, Keene A, Zhang LW, Bancos S, Knapton A. Nanoparticle toxicity predictions using an in vitro systems-biology approach (Final Report). Funding: Critical Path 2010.

Mentees:

Mentees

1. Jingling Li, M.S. xxxxx xxxxx xxxxx

2. Yanglie Cheng, M.S. xxxxx xxxxx xxxxx

Journal Reviewer

Awards and Scholarships                                            

2009 --Battelle Memorial Research Award, the 48th Annual Meeting of the Society of Toxicology, Baltimore, MD, 2009

2008 -- North Carolina State University Graduate School Travel Award  

2008 --Graduate Association Travel Award at the college of veterinary medicine of North Carolina State University  

2008 -- Award for Best Student at the In Vitro and Alternative Methods Specialty Section at the 47th Annual Meeting of the Society of Toxicology, Seattle, WA

2008 -- First place award for the MB Research Award, at the 47th Annual Meeting of the National Society of Toxicology, Seattle, WA  

News Reports

Quantum Dots Can Penetrate Skin Through Minor Abrasions. Science Daily. May 11, 2009, from http://www.sciencedaily.com/releases/2008/07/080702103327.htm

Also the reports are available at Biology News Net, Biospace, Medical News Today, Nanotechnology Today, Nanotechnology Now, RxPG News, Nanotechwire.com, Cosmetics and Toiletries.com, Red Orbit, AZoNanotechnology, The Medical News, NCSU News Release, NANO@NC STATE.

Professional Associations and activities

2006 – Present  Membership in Society of Toxicology

2009 – Present Full membership, Sigma Xi Scientific Research Society

2011 – 2012       Officer, Nanotoxicology Specialty Section, Society of Toxicology

2012 – 2015      Education Committee Officer, Society of Toxicology